The University of Applied Sciences in Utrecht investigates 2D organoids of the intestine. These organoids are cultured in a conventional 12 well transwell with inserts. With the Artemis the growth of the Organoids will be monitored over a period of approximately 3 weeks, while the organoids remain in the incubator. With this organoid intestine model, the influence of food on the hormone excretion in the gut is investigated. The Artemis performs TEER / impedance measurements according to following requirements:
TEER measurements in 12 wells
Adjustable regular time interval in between TEER measurements
Runtime at least 3 weeks
Adjustable flow rate of cell culture media circulation
Flow can be adjusted to timing of the TEER measurements
Figure 2. Adapter to 12 well transwell.
Figure 1. Organoids of the intestine, courtesy of Jean-Paul ten Klooster, University of Applied Sciences.